By John M. Davis
It is a accomplished examine advisor that describes either the most important new innovations and extra validated equipment. each bankruptcy discusses the benefits and barriers of a number of the ways after which offers chosen tried-and-tested protocols, in addition to a plethora of excellent sensible recommendation, for instant use on the bench. It provides the main obtainable and finished creation to be had to the tradition and experimental manipulation of animal cells. designated protocols for a wide selection of tools give you the center of every bankruptcy, making new technique simply accessible.This ebook is a necessary laboratory handbook for all undergraduates and graduates approximately to embark on a phone tradition undertaking. it's a publication which either skilled researchers and people new to the sphere will locate important.
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Additional info for Animal Cell Culture: Essential Methods
Humidity The atmosphere within the incubator is normally humidified by means of a tray of water placed in the bottom of the incubator, with a fan ensuring even distribution of humidity around the chamber. De-ionized or RO water should be used, with a regular and frequent preventative maintenance schedule in place to ensure the tray is emptied, thoroughly cleaned and refilled, as it can potentially be a source of bacterial and fungal contamination. It is therefore important that the tray can be easily accessed.
However, whenever possible the use of single-use disposable plasticware is highly recommended over the alternative method of recycling and reusing glassware. Glassware has a propensity for adsorbing substances such as alkaline detergent onto its surface. If glassware is to be used it needs to be of a high-quality borosilicate and supported by the use of good washing facilities. There is no doubt that plastic containers are less prone to leaching trace elements that may be deleterious to cells. 1 Tissue culture plasticware There are a number of companies that can offer the standard items of sterile cultureware required for tissue culture at comparable cost and ISO9001-certified quality.
R Trolleys: may be useful for the transportation of large volumes of media and waste. r Cell freezing device: providing controlled rate freezing for the long-term storage of cells (see Chapter 6). r Weighing balance, magnetic stirrers: for preparation of media and reagents. r pH meter and osmometer: to check pH and osmolarity of media. r Automated cell culture instrumentation: for example cell counters, or spent medium analysers to assess culture growth. 4 Culture plasticware and associated small consumable items Laboratories differ in their preference for the use of glass or plasticware for cell culture and related operations.
Animal Cell Culture: Essential Methods by John M. Davis