Download e-book for kindle: Angiogenesis Protocols by Stewart Martin, Cliff Murray

By Stewart Martin, Cliff Murray

ISBN-10: 0896036987

ISBN-13: 9780896036987

ISBN-10: 1592591434

ISBN-13: 9781592591435

Numerous anti-angiogenic brokers are actually in medical trials for quite a number illnesses characterised through out of control blood vessel formation. This quantity presents a suite of up to date reports at the very important elements of this technique in addition to numerous strategies for learning angiogenesis.

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And Ellis, L. M. (1995) Expression of vascular endothelial growth factor and its receptor, KDR, correlates with vascularity, metastasis and proliferation of human colon cancer. Cancer Res. 55, 3964–3968. Brown, L. , Jackman, R. , Guidi, A. , Dvorak, H. , et al. (1995) Expression of vascular permeability factor (vascular endothelial growth factor) and its receptors in breast cancer. Hum. Pathol. 26, 86–-91. , Zhang, H. , Fan, T. , Hu, D. , Lees, V. , et al. (1995) Thymidine phosphorylase is angiogenic and promotes tumor growth.

J. (1988) Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures. J. Cell Biol. 107, 1589–1598. 3. Grant, D. , Kinsella, J. , Piasecki, B. , and Kleinman, H. K. (1992) Interaction of endothelial cells with a laminin A chain peptide (SIKVAV) in vitro and induction of angiogenic behavior in vivo. J. Cell Physiol. 153, 614–625. 4. , Taylor, R. , Long, P. , Haney, J. , Pauly, R. , Grant, D. , and Martin, G. R. (1992) A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor.

8. Endothelial cell growth supplement [bovine brain extract containing acid fibroblast growth factor (aFGF) and bFGF (ECGS)] (Collaborative Biomedical, 50 Malinda 9. 10. 11. 12. Becton-Dickinson, Rutherford, NJ): Prepare 1 mg/mL solution just before use in sterile, distilled H20 and store unused portion in aliquots at –20°C. ) Or bFGF (Collaborative Biomedical, Bedford, MA). Prepare solution 1 mg/mL just before use in sterile, distilled H20 and store unused portion in aliquots at –20°C. Final concentration used in experiment is either 10 ng/mL or 100 ng/mL ECGS or 150 ng/mL bFGF.

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Angiogenesis Protocols by Stewart Martin, Cliff Murray


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